Metapangenomic investigation provides insight into niche differentiation of methanogenic populations from the subsurface serpentinizing environment, Samail Ophiolite, Oman

Serpentinization reactions produce highly reduced waters that have hyperalkaline pH and can high concentrations of H 2 CH 4 . Putatively autotrophic methanogenic archaea been identified in the subsurface Samail Ophiolite, Sultanate Oman, though strategies to overcome dissolved inorganic carbon limitation remain be fully understood. Here, we recovered metagenome assembled genomes (MAGs) applied a metapangenomic approach three different Methanobacterium populations assess habitat-specific functional gene distribution. A Type I population was fluids with neutral pH, while II “Mixed” were most (pH 11.63). The core genome all highlighted potential DNA scavenging techniques phosphate or nitrogen induced by environmental conditions. With particular emphasis on Mixed found fluids, accessory unique each reflected adaptation mechanisms suggesting lifestyles minimize niche overlap. In addition previously reported metabolic capability utilize formate as an electron donor generate intracellular CO , possessed genes relevant defense against antimicrobials assimilating osmoprotectants provide cellular stability. enriched for multiple glycosyltransferases energetic costs adhering mineral surfaces other microorganisms, fostering non-motile lifestyle. These results highlight differentiation distinct circumvent challenges serpentinization impacted through coexistence strategies, supporting our ability understand controls adaptations within serpentinizing Ophiolite.